DNA

Part:BBa_K4769109:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


Porphyran utilisation locus fragment 3 integration sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 625
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 655


Design Notes

N/A


Source

P. plebeius genome

Primers for cloning: FW: gtatcgacatggctttatataataacaagc (+ desired overhangs) RV: ttagaaattaatctggaatccgccac (+ desired overhangs)

References